Cellix App Note C300: Application note investigating monocyte adhesion to chemokines and the effect of oxidized LDL treatment to HUVECs on the adhesion profile - all under physiologically relevant shear stress conditions.  Microchannels of Cellix's biochips were coated with different ligands/chemokines or cultured endothelial cells and Cellix's microfluidic pumps were used to flow THP1 or PBMC over the surface at different shear rates. The inhibition profile of THP1 adhesion to VCAM-1 was measured using an anti-α4 mAb to block the α4β1 (VLA-4) receptor for VCAM-1 and the THP1 adhesion profile to TNFα or IFNγ (inflammatory cytokines) stimulated endothelial cells was measured.  Effect of oxLDL on THP1 adhesion to endothelial cells was also measured. The inhibition profile of PBMC adhesion to Fracktalkine (Fkn) was measured using the soluble form of Fkn (chemokine domain) to block the CX3CR1 receptor for Fkn.  The effect of MCP-1 on PBMC adhesion to VCAM-1 and Fractalkine was also investigated.

  Cellix App Note I100Application note investigating the effect of a range of statins (pravastatin, fluvastatin, mevastatin, lovastatin, simvastatin) on T-cell adhesion to ICAM-1 under physiological shear stress / flow conditions in a mixed sepsis model.  Microchannels of Cellix's biochips were coated with rhICAM-1 and Cellix's microfluidic pumps were used to flow T-cells, co-cultured with monocytes and pre-stimulated with LPS/PepG, over the surface at different shear rates.

  Cellix App Note I200: Application note investigating the role of different adhesion molecules and chemokines involved in various stages of inflammation under physiological flow.  Microchannels of Cellix's biochips were coated with rhVCAM-1, E-selectin or cultured endothelial cells (HUVECs; non stimulated and TNFα treated) and Cellix's microfluidic pumps were used to flow THP1, monocytes and PBMCs (treated with anti-VCAM-1 mAb, anti-E-selectin mAb) over the surface at different shear rates.  Experiments were repeated to investigate the effect of MCP-1 (monocyte chemoattractant protein) stimulation on the adhesion profiles.

  Cellix App Note O100: Application note investigating the mechanism by which melanoma cells metastasise with a study of differential cell adhesion within an isogenic model of melanoma progression under physiological shear flow conditions.  Microchannels of Cellix's biochips were coated with rhICAM-1, rhVCAM-1, fibronectin or cultured endothelial cells (HUVECs) and Cellix's microfluidic pumps were used to flow the melanoma cell lines 1205Lu, WM793, WM793-P1 and WM793-P2 over the surface at different shear rates.

  Cellix App Note R100Application note investigating the novel anti-inflammatory effects of Montelukast (MLK; class of anti-asthma drugs that are antagonists to cysLT1R reducing eosinophil migration) on the adhesion profile of resting and GM-CSF-stimulated eosinophils to rhVCAM-1 under physiological shear flow conditions.  Microchannels of Cellix's biochips were coated with rhVCAM-1 and Cellix's microfluidic pumps were used to flow the eosinophils over the surface at different shear rates.  The effect of MLK on the adhesion profile of eosinophils to rhVCAM-1 was compared against MK571, leukotriene biosynthesis inhibitor MK886 and an anti-CysLT1R antibody

  Cellix App Note R200Application note investigating the effect of levocetirizine on human eosinophil adhesion to rhVCAM-1 under physiological shear flow conditions.  Microchannels of Cellix's biochips were coated with rhVCAM-1 and Cellix's microfluidic pumps were used to flow the eosinophils over the surface at different shear rates.