Cellix App Note C300: Application note investigating monocyte adhesion to chemokines and the effect of oxidized LDL treatment to HUVECs on the adhesion profile - all under physiologically relevant shear stress conditions. Microchannels of Cellix's biochips were coated with different ligands/chemokines or cultured endothelial cells and Cellix's microfluidic pumps were used to flow THP1 or PBMC over the surface at different shear rates. The inhibition proﬁle of THP1 adhesion to VCAM-1 was measured using an anti-α4 mAb to block the α4β1 (VLA-4) receptor for VCAM-1 and the THP1 adhesion profile to TNFα or IFNγ (inflammatory cytokines) stimulated endothelial cells was measured. Effect of oxLDL on THP1 adhesion to endothelial cells was also measured. The inhibition proﬁle of PBMC adhesion to Fracktalkine (Fkn) was measured using the soluble form of Fkn (chemokine domain) to block the CX3CR1 receptor for Fkn. The effect of MCP-1 on PBMC adhesion to VCAM-1 and Fractalkine was also investigated.
Cellix App Note I200: Application note investigating the role of different adhesion molecules and chemokines involved in various stages of inﬂammation under physiological ﬂow. Microchannels of Cellix's biochips were coated with rhVCAM-1, E-selectin or cultured endothelial cells (HUVECs; non stimulated and TNFα treated) and Cellix's microfluidic pumps were used to flow THP1, monocytes and PBMCs (treated with anti-VCAM-1 mAb, anti-E-selectin mAb) over the surface at different shear rates. Experiments were repeated to investigate the effect of MCP-1 (monocyte chemoattractant protein) stimulation on the adhesion profiles.
Cellix App Note O100: Application note investigating the mechanism by which melanoma cells metastasise with a study of differential cell adhesion within an isogenic model of melanoma progression under physiological shear flow conditions. Microchannels of Cellix's biochips were coated with rhICAM-1, rhVCAM-1, fibronectin or cultured endothelial cells (HUVECs) and Cellix's microfluidic pumps were used to flow the melanoma cell lines 1205Lu, WM793, WM793-P1 and WM793-P2 over the surface at different shear rates.